Introduction: extreme or extended glucocorticoid (GC) management may lead to high risk of bone fractures and osteoporosis (OP). Alpha lipoic acid and Royal Jelly are effective anti-oxidants. Objective: This study examined effects of glucocorticoid on the bone structure and the possible preventive role of alpha Lipoic acid and royal jelly. Materials and Methods: Forty rats were categorized into four groups. Control group, corticosteroid group: rats were administrated with glucocorticoid at a dose of 30 mg/kg/day S.C for 60 days, third group rats were given Glucocorticoid and 100 mg/kg/day of alpha lipoic acid orally for 60 days. Group IV: rats were given glucocorticoid and royal jelly 100 mg/Kg /day orally for 2 months. Bone specimens were prepared for the histological and immunohistochemical studies. Results: Glucocorticoid induced resorption and damage of the bone histological structure. Percent of both collagen fibre deposition and osteopontin immunoreactivity were markedly decreased in comparison with the control group. Royal jelly and alpha lipoic acid reversed the damage effect of glucocorticoid on the bone histological structure. Conclusion: Chronic use of glucocorticoid in adult male albino rats caused osteoporosis that could be reversed by administration of royal jelly and alpha lipoic acid.