Angka kejadian Hepatitis B masih sangat tinggi di dunia dan Indonesia, perjalanan penyakit secara kronis menjadi kanker hati. Kejadian Hepatitis B memiliki banyak faktor risiko seperti faktor sosiodemografi, gaya hidup dan golongan darah. Tujuan penelitian adalah menganalisis pengaruh faktor sosiodemografi dan golongan darah terhadap kejadian hepatitis B. Jenis penelitian adalah penelitian retrosprektif dari tahun 2018-2020. Subjek penelitian sebanyak 100 orang. Teknik pengambilan data dengan teknik simple random sampling dari data rekam medis PMI Kota Bengkulu. Hasil penelitian menunjukkan jenis kelamin laki-laki sebanyak 84 orang (84%) dan perempuan sebanyak 16 orang (16%). Usia subjek penelitian paling banyak ada pada rentang 20-29 tahun (43%). Pekerjaan yang terbanyak mengalami infeksi hepatitis B adalah wiraswasta (28%). Golongan darah yang paling banyak mengalami infeksi Hepatitis B adalah golongan darah A+ (41%). Analisis multivariat dengan metode PCA menunjukkan secara statistik bahwa faktor eksternal (faktor pekerjaan) dan faktor internal (golongan darah, usia dan jenis kelamin) mempengaruhi kejadian Hepatitis B sebesar 36,3% dan  27,5%. Hasil regresi logistik menunjukkan terdapat pengaruh faktor eksternal dan internal terhadap kejadian Hepatitis B sebesar 26%. The incidence of Hepatitis B is still very high globally, and in Indonesia, the chronic manifestations become liver cancer. The incidence of Hepatitis B has many risk factors such as sociodemographic factors, lifestyle, and blood type. The research aimed to analyze the influence of sociodemographic factors and blood type on the incidence of hepatitis B. This study was a retrospective study from 2018-2020. The research subjects were 100 people. The data collection technique used a simple random sampling technique from the medical record data of PMI Bengkulu City. The results showed 84 men (84%) and 16 women (16%). The age of most research subjects is in the range of 20-29 years (43%). The occupation with the most hepatitis B infection is entrepreneurs (28%). The blood group with the most hepatitis B infection was blood type A+ (41%). Multivariate analysis using the PCA method showed statistically that external factors (occupational factors) and internal factors (blood type, age, and sex) affected the incidence of Hepatitis B by 36.3% and 27.5%, respectively. The logistic regression results showed an influence of external and internal factors on the incidence of Hepatitis B by 26%.

This study aims to determine the effect of administration of Etlingera hemispharica leaf extract on white rats (Rattus norvegicus) induced by mercury chloride as seen from the histopathological description of the testes. The research method used is experimental post-test-only control group design. The extract was administered for seven days by gavage. Testicular organ harvesting was carried out on the 9th day after the rats were euthanized and continued with making testicular preparations. Testicular damage was assessed using the Johnsen score. Data were analyzed using non-parametric statistical tests. The results showed that the test groups had a p-value of 0.000. The histopathological description of the testes induced by HgCl2 had a p-value of 0.005 and the administration of E. hemisphaerica leaf extract in HgCl2-induced mice showed a p-value of 0.008. In conclusion, administration of E. hemisphaerica leaf extract to R. norvegicus induced by mercury chloride improved spermatogenesis on the histopathological features of the R. norvegicus testes. Keywords: Etlingera hemispharica, HgCl2, Johnsen Score, Rattus norvegicus

Kegiatan pengabdian dilaksanakan dikarenakan sering terjadi kelangkaan gas sehingga harga gas menjadi tinggi. Kelangkaan dan tingginya harga gas menyebabkan masyarakat kembali beralih ke kayu untuk keperluan memasak sehari-hari. Sementara Kelonpok Tani Karya Bersama mempunyai potensi yang besar berupa limbah kotoran sapi yang dapat digunakan sebagai penghasil biogás. Biogas dapat dimanfaatkan untuk memasak seharihari. Pemanfaatan kotoran sapi menjadi biogás belum dilakukan karena tidak ada sumber daya manusia yang mampu mengolah kotoran sapi tersebut. Untuk itu, dilakukan pelatihan embuatan dan pengoperasian instalasi biogás sistem kontinu pada Kelompok Tani Karya Bersama, RT 15 Kandang Limun, Kota Bengkulu. Pelatihan dilaksanakan pada Kelompok Tani ini sebagai contoh bagi masyarakat lainnya. Kelompok tani ini telah berhasil membuat dan mengoperasikan instalasi biogas, sehingga ke depan diharapkan menjadi fasilitator munculnya instalasi-instalasi baru dan masyarakat peternak sapi tidak bergantung lagi pada gas dan minyak tanah.

Introduction: Carotid intima-media thickness (CIMT) is a predictor marker of subclinical atherosclerosis that can be measured by ultrasound technique. The clinical symptoms of atherosclerosis usually begin with the thickening of the carotid artery walls. Polymorphism in Apolipoprotein E4 (APO-E4) is considered to be an important genetic determinant of atherosclerosis and Coronary Artery Disease (CAD). The genetic role of APO-E4 on plasma lipid has shown that the APO-E4 allele is associated with increased plasma LDL and total lipid concentration. The main objectives of this study were to identify genotype polymorphisms of the APO-E gene and measure the thickness of the carotid intima in subjects with APO-E4 polymorphisms in the first descendant of coronary heart patients. Patients and methods: A cross-sectional study design was used to determine the polymorphism of the APO-E4 genotype in the first descendant of CAD and non-CAD groups. There were 21 samples in each group. Gene polymorphism examination was carried out by PCR and the CIMT was measured with doppler ultrasound. Results: The result showed that 21.4% of subjects had polymorphism of the APO-E4 genotype with a significant difference between CHD and non-CHD group. This data shows that the APO-E4 genotype is more dominant in the first descendant of patients with CHD when compared to non-CHD parents. The mean values of CIMT ini right and left-sided carotid arteries among APO-E4 genotype and non-APO-E4 genotype were 0.4743 mm vs. 0.4195 mm and bivariate analysis showed a significant difference with a P-value of 0.017. This shows that there is a significant difference in the thickness of the carotid intima in the first descendant of subjects who have the APO-E4 genotype and those who do not. Conclusion: The APO-E4 gene polymorphism was more dominant in the first descendant of CAD patients with a higher CIMT mean value.

This study aims to identify the VEGF-A gene polymorphism (rs2010963) in the first generation of patients with coronary heart disease (CHD) and non-CHD in Bengkulu City. The method used is molecular analysis of polymorphism through Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP). This type of research is descriptive observational with a cross-sectional research design. The results of this study indicate that the genotype frequency of the VEGF-A gene polymorphism (rs2010963) in the first generation of patients with CHD is genotype CC = 11 (52.4 %), GG = 7 (33.3%) and CG = 3 (14.3%). ). The distribution of the VEGF-A genotype (rs2010963) in the first non-CHD offspring, namely GG = 9 (42.9%), CC = 7 (33.3%) and GC = 5 (23.8%). The statistical analysis results showed that the p-value obtained was 0.460 for the VEGF-A gene polymorphism genotype (rs2010963) in the first CHD and Non-CHD derived samples. In conclusion, there was no significant difference in the VEGF-A gene polymorphism in the first generation of CHD and non-CHD groups in Bengkulu City. Keywords: PCR-RFLP, Coronary Heart Disease, Gene Polymorphism, VEGF-A