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The aim of this study was to develop an integrated biofuel (cellulosic bioethanol) and biochemical (succinic acid) production process from rapeseed straw after dilute-acid pretreatment. Rapeseed straw pretreatment at 20% (w/v) solid loading and subsequent hydrolysis with Cellic® CTec2 resulted in high glucose yield (80%) and ethanol output (122-125 kg of EtOH/Mg of rapeseed straw). Supplementation the enzymatic process with 10% dosage of endoxylanases (Cellic® HTec2) reduced the hydrolysis time required to achieve the maximum glucan conversion by 44-46% and increased the xylose yield by 10% compared to the process with Cellic® CTec2. Significantly higher amounts of succinic acid were produced after fermentation of pretreatment liquor (48 kg/Mg of rapeseed straw, succinic acid yield: 60%) compared to fermentation of xylose-rich residue after ethanol production (35-37 kg/Mg of rapeseed straw, succinic yield: 68-71%). Results obtained in this study clearly proved the biorefinery potential of rapeseed straw.