Auxin (IAA) is versatile signalling molecule of plants, currently classified as plant hormone. But there are data suggesting that auxin is acting also as plant-specific morphogen, electric-responses inducing transmitter, and as general signalling molecule used for plant-bacteria communication. Our previous data revealed that auxin is associated with secretory endosomes and also highly enriched within cell walls of cells active in transcellular auxin transport. Our present data, based on in vivo non-invasive auxin flux recordings, reveal that auxin is secreted out of synaptic-like domains specialized for efflux of auxin in root apex cells highly active in polar cell-cell transport of auxin. We obtained both genetic and pharmacological evidence that phospholipase Dzeta2 drives vesicular secretion of auxin for its polar transcellular transport in the transition zone of the root apex. Secretion of auxin via secretory vesicles has far-reaching consequences not only for our understanding of cell-cell auxin transport but also for plant sciences as a whole.