Anthropological research indicates that Brosimum alicastrum seed, also known as Ramón tree or mojo was one of the staple foods of pre‐Columbian people. Because of world population growth demand alternative source of food, there is an increasing use of B. alicastrum, especially in American continent. In order to contribute to its rational use, this research presents, biochemical and functional characterization of mojo albumins and globulins. Our results demonstrated that these storage proteins are the most abundant protein fractions. Albumins presented good solubility at pH values of 4–9; the best emulsifying activity index (EAI) was found at pH 7 (322.93 m2/g). Globulins showed a high solubility at pH 2 and at a pH range of 5–9; the maximum EAI values (339.30–309.88 m2/g) were found at pH 4–5. Practical applications Brosimum alicastrum seeds have been used a food source since the pre‐Colombian period, however, there is no information about their proteins (albumins, globulins, prolamins, and glutelins). We have found that mojo seeds can be employed as an alternative source of gluten‐free food and can also be used in the development of novel and/or conventional food products because of biochemical and functional properties of their albumins and globulins, main storage proteins.

Triclosan (TCS) is used in many health care products as a broad-spectrum antimicrobial and disinfectant agent; however, it is currently considered an emerging pollutant. The present study aimed to develop an analytical HPLC method coupled to a Diode Array Detector for the TCS determination in toothpaste. Method development involved the optimization of separation and sample preparation. For chromatographic separation optimization, different RP columns and aqueous mobile phases were addressed. The optimized chromatographic separation was achieved in around 3 minutes using a Symmetry C18 column at 30 °C, 0.6 mL/min flow-rate, and 225 nm wavelength detection. For toothpaste sample preparation optimization a response surface methodology using a Box-Behnken fractional factorial design was chosen. The sample treatment consisted of leaching 0.2 g sample by sonication for 7 minutes using 2 mL of MeOH. According to the International Conference on Harmonization (ICH, 1996/2005) and European Commission Decision 200/657/EC guidelines, the developed method was validated. The developed analytical method was found fast, economic, linear, sensitive, precise, and robust and allowed to quantify TCS in different healthcare products.

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