Burnout is a variable that continues to sustain international research interest. The most widely adopted tool measuring the burnout syndrome is the Maslach Burnout Inventory (MBI). The present study attempted to examine the psychometric properties and factorial structure of the MBI amongst a sample of 536 professional health specialists in Greece. The results indicated satisfactory reliability through internal consistency for all three dimensions. However, a factor analysis did not produce conclusive evidence of a tripartite construct, but rather the predominance of a single primary factor (emotional exhaustion), and a secondary factor (depersonalization). The results confirm previous findings advocating the uni-factorial or bi-factorial nature of the construct.