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A rapid fluorescence flow injection immunoassay has been developed for a model analyte (testosterone) using a novel perfusion chromatographic matrix (POROS) coated with protein A. The fluorophore used was Texas Red. The perfusion matrix contains two classes of pores, which radically enhances mass transport and provides a very high capacity unaffected by flow rate. For each assay the antibody-protein A reaction takes place at nearly neutral pH, and the complexes are eluted at acidic pH. Both preincubation and on-line assay formats were investigated. The total time for the pre-incubation and on-line assays was less than 4 and 6 min, respectively. Serum testosterone was determined by this method and good relative standard deviations and recoveries were achieved. The use of an avidin-biotin system in the preparation of the testosterone Texas Red conjugate is also described.

Detector An immunoassay , has been developed for phenytoin in serum. The assay involves flow injection with fluorescence detection and use of Poros A material as the solid phase. valve Paper 3f06316 J

A new long-wavelength fluorigenic substrate for alkaline phosphatase: synthesis and characterisation C o m m u n i c a t i o n

No abstract