Population growth and climate change challenge our food and farming systems and provide arguments for an increased intensification of agriculture. A promising option is eco-functional intensification through organic farming, an approach based on using and enhancing internal natural resources and processes to secure and improve agricultural productivity, while minimizing negative environmental impacts. In this concept an active soil microbiota plays an important role for various soil based ecosystem services such as nutrient cycling, erosion control and pest and disease regulation. Several studies have reported a positive effect of organic farming on soil health and quality including microbial community traits. However, so far no systematic quantification of whether organic farming systems comprise larger and more active soil microbial communities compared to conventional farming systems was performed on a global scale. Therefore, we conducted a meta-analysis on current literature to quantify possible differences in key indicators for soil microbial abundance and activity in organic and conventional cropping systems. All together we integrated data from 56 mainly peer-reviewed papers into our analysis, including 149 pairwise comparisons originating from different climatic zones and experimental duration ranging from 3 to more than 100 years. Overall, we found that organic systems had 32% to 84% greater microbial biomass carbon, microbial biomass nitrogen, total phospholipid fatty-acids, and dehydrogenase, urease and protease activities than conventional systems. Exclusively the metabolic quotient as an indicator for stresses on microbial communities remained unaffected by the farming systems. Categorical subgroup analysis revealed that crop rotation, the inclusion of legumes in the crop rotation and organic inputs are important farming practices affecting soil microbial community size and activity. Furthermore, we show that differences in microbial size and activity between organic and conventional farming systems vary as a function of land use (arable, orchards, and grassland), plant life cycle (annual and perennial) and climatic zone. In summary, this study shows that overall organic farming enhances total microbial abundance and activity in agricultural soils on a global scale.

Highly adapted herbivores can phenocopy two-component systems by stabilizing, sequestering and reactivating plant toxins. However, whether these traits protect herbivores against their enemies is poorly understood. We demonstrate that the western corn rootworm Diabrotica virgifera virgifera, the most damaging maize pest on the planet, specifically accumulates the root-derived benzoxazinoid glucosides HDMBOA-Glc and MBOA-Glc. MBOA-Glc is produced by D. virgifera through stabilization of the benzoxazinoid breakdown product MBOA by N-glycosylation. The larvae can hydrolyze HDMBOA-Glc, but not MBOA-Glc, to produce toxic MBOA upon predator attack. Accumulation of benzoxazinoids renders D. virgifera highly resistant to nematodes which inject and feed on entomopathogenic symbiotic bacteria. While HDMBOA-Glc and MBOA reduce the growth and infectivity of both the nematodes and the bacteria, MBOA-Glc repels infective juvenile nematodes. Our results illustrate how herbivores combine stabilized and reactivated plant toxins to defend themselves against a deadly symbiosis between the third and the fourth trophic level enemies.

The majority of soil organic nitrogen (N) is bound in protein-like compounds and therefore its proteolysis in peptides and amino acids is considered the initial and rate limiting step of N mineralization. Proteolysis of N bound in organic fertilizer and subsequent provisioning for crops is a central element in agro-ecological intensification. Long-term farming system effects on N provisioning from organic fertilizer to crops and its underlying functional microbial communities were analyzed in experiments conducted in soils from the "DOK" system comparison trial (bio-Dynamic, bio-Organic, and "Konventionell") subjected to optimal and future projected drought scenarios. A plant nutrition experiment using 15 N labeled lupine as a fertilizer (green manure) identified 30% higher amounts of N derived from fertilizer in ryegrass grown on organically compared to conventionally managed soil, but only when subjected to dry conditions. A second experiment, also amended with lupine green manure, assessed the effect of farming system and drought stress on N cycling microbes with a focus on alkaline (apr) and neutral (npr) metallopeptidase encoding microbial communities. apr encoding microbial communities were more strongly affected by farming system and water treatment than npr encoding communities. Differences in structure and diversity of apr encoding microbial communities showed concomitant patterns with distinct N provisioning from organic fertilizer in the plant nutrition experiment. It is suggested that conventionally managed systems are less capable in maintaining diversity and initial structure of apr encoding microbial communities when subjected to drought scenarios. Overall, we demonstrated organically managed soils to provide a more stable N provisioning potential from organic fertilizer under future drought scenarios, likely facilitated by a distinct and more adaptive proteolytic microbial community. This work contributes to an in-depth comprehension of yet poorly studied fundamental soil processes and helps developing strategies to maintain a versatile and functioning microbial community in a rapidly changing environment.