This article reviews studies about the effects of radiofrequency electromagnetic (RF EM) fields on male reproductive system and reproductive health in mammals. According to current data, there are almost 4 million active mobile phone lines in Croatia while this number has risen to 2 billion in the world. Increased use of mobile technology raises scientific and public concern about possible hazardous effects of RF fields on human health. The effects of radiofrequencies on reproductive health and consequences for the offspring are still mainly unknown. A number of in vivo and in vitro studies indicated that RF fields could interact with charged intracellular macromolecular structures. Results of several laboratory studies on animal models showed how the RF fields could affect the mammalian reproductive system and sperm cells. Inasmuch as, in normal physiological conditions spermatogenesis is a balanced process of division, maturation and storage of cells, it is particularly vulnerable to the chemical and physical environmental stimuli. Especially sensitive could be the cytoskeleton, composed of charged proteins; actin, intermedial filaments and microtubules. Cytoskeleton is a functional and structural part of the cell that has important role in the sperm motility, and is actively involved in the morphologic changes that occur during mammalian spermiogenesis.