Nurses are responsible for the holistic care of substance use disorder patients, which encompasses the psychosocial, developmental, cultural, and spiritual needs of the individual, and helped to manage physical needs, prevent illness, and treat health conditions, they consider the vital caregiver for SUD patients. To do this important role, they need to have good knowledge, positive attitude and caring behavior skills toward these patients. Aim of the study: This study was aimed to determine the effect of training program on the nurses' attitude and perception of caring behavior toward substance use disorder patients. Subjects of study: Sample consisted of 50 nurses who were chosen by convenient sampling. A quasi-experimental design was utilized. Tools of the study: Two tools were used for data collection; Substance Abuse Attitudes Survey (SAAS) designed to measure nurses' attitudes towards substance use disorder and Caring Behavior Assessment Tool (CBA) aims to assess nurse' perception regarding caring behavior toward substance use disorder patients. Result of the study: The results revealed that there was statistically significant improvement in nurses' attitude and perception of caring behavior skill regarding substance use disorder patients before and after implementing of the training program. Conclusion , recommendation: The study concluded that training program about substance use disorder enhancing the nurses' attitude and perception of caring behavior skills toward substance use disorder patients .According to these results, the study recommended that hospital policies must encourage nurses to attend in-service training program about attitude and caring behavior skills toward substance use disorder patients.