Background: Safe patient handling and movement is very important part of the organizational culture. It is basic element and guide to improve nurse knowledge. Aim of the study: Evaluate the effect of nursing guidelines on orthopedic nurses' knowledge regarding safe patient handling and movement. Design: Quasi-experimental research design was utilized in this study. Setting: This study was conducted in orthopedic departments at Mansoura University Hospital. Subject: All available number of nurses (50) working in orthopedic department. Tools: One tool structure questionnaire sheet was used in this study. Results: The present study revealed that more than half of studied nurses' had fair knowledge pre intervention compared to above two third had good knowledge post intervention. Conclusion: It can be concluded that there was a positive effect of nursing guidelines on improving nurses' knowledge regarding safe patient handling and movement. Recommendations: This study recommended the availability of written guidelines regarding safe patient handling and movement in orthopedic department.