This study evaluated the protective effect of Sukkari date extract on Streptozotocin(STZ)induced diabetes in rats. Forty male albino rats were divided into 2 main groups: Group I (Control group, n=10): received no drugs and served as control. Group II (Diabetic group, n=30):received a single dose of STZ (50mg/kg b.wti.p.) for induction of diabetes, and 48hoursafterdiabetes induction,the rats were divided into 3 equal subgroups. Subgroup (a): Diabetic non-treated rats.Subgroup (b): Diabetic aqueous sukkari date extract-treated rats.Subgroup (c): DiabeticAmaryl-treated rats. Treatment with sukkari date extract(10 ml/rat/day) and Amaryl(1ml/rat/day) were given orally and daily for 60 days. Blood samples and liver tissues were collected from all animals once after 60 days of treatment for determination of serum glucose, lipids profile, insulin,L-malondialdehyde (L-MDA), superoxide dismutase (SOD) and 8-hydroxy-2-deoxyguanosine (8-OHdG) inaddition to paraoxonase 1 (PON1) gene expression level in liver tissue. The obtained results showed a significant increase in serum glucose, total cholesterol, triacylglycerols, low density lipoprotein-cholesterol (LDL-c), L-MDA and 8-OHdG levels with marked decrease in serum insulin, high density lipoprotein-cholesterol (HDL-c) and SOD activity in addition to significant down-regulation of PON1 gene expression level in liver in STZ-induced diabetic rats.On the other hand, treatments of diabetic rats with aqueous sukkari date extract or amaryl exhibited a significant ameliorative effect on all previous biomarkers and approximately reach to normal levels. This study suggest the ameliorativerole of aqueous sukkari date extract in diabetes and its protective effect may be mediated through reduction in oxidative stress, the promising hypoglycemic and hypolipidemic activity.