No abstract

a considerable native fluorescence in methanol was measured at 310 nm (ex. 230 nm). All variables affecting reaction conditions were optimized. Linear correlations were obtained over the range of 19-100, 37-400 and 22-150 ng/ml for (I), (III) and (IV), respectively. The proposed methods were successfully applied for the analysis of the studied drugs in their pure and commercial dosage forms and the obtained results were in good agreement with those obtained from the reported methods; no significant difference in the accuracy and precision as revealed by the accepted values of t-and F-tests, respectively.

Two simple and sensitive spectrophotometric methods were developed for the determination of paracetamol (I) and ascorbic acid (II) in pharmaceutical binary mixture. The first method depends on the use of the first-derivative spectrophotometric technique for the simultaneous determination of components of the mixture. The second method depends on the reaction of the studied drugs with 5-diazo-1, 2, 4-triazol-3-carboxylic acid (DTCA) reagent to give colored products measured at 480 nm and 380 nm for (I) and (II), respectively. All variables affecting reaction conditions were optimized. The proposed methods were successfully applied for the analysis of the studied drugs in their pure and commercial dosage forms and are in good agreement with those obtained from the reported methods. No significant difference in the accuracy and precision as revealed by the accepted values of t-and F-tests, respectively. Molar ratios of the drugs with the colorimetric reagent (DTCA) were determined and the reaction mechanisms were suggested.

Autism spectrum disorders are a neurodevelopmental condition characterized by disturbances in social interaction and communication, as well as repetitive and restricted behaviors. No efficient treatment plans have been found so far. The neurodevelopmental effects of antiandrogens on developmental milestones were examined in rats following a single intraperitoneal injection of adult pregnant female rats with valproic acid (VPA) (600 mg/kg) on gestation day 12.5. Control females were injected with physiological saline at the same time. The offspring of set 1 (which received physiological saline) was divided into 2 groups (groups 1&2) and the offspring of set 2 (which received VPA) were divided into 5 groups (groups 3-7). Thus, the seven groups were organized as follows: Group 1, the control group in which pups did not receive any treatment. Group 2, pups were given sesame oil vehicle. Group 3 pups did not receive any further treatment. Group 4 in which the pups were given sesame oil. Group 5 in which the pups received 1 mg/day testosterone propionate subcutaneously. Group 6 in which the pups received flutamide 1 mg/kg subcutaneously. Group 7 in which the pups received finasteride 50 mg/kg subcutaneously. Our results demonstrated that VPA-induced retardation in sensorimotor reflexes, but did not affect physical landmarks while antiandrogens improved developmental milestones. In conclusion, our finding confirms that androgen contributes partially towards VPA-induced neurodevelopmental insults.