In present scenario, ecofriendly approach for the synthesis of noble metals nanoparticles by means of various plant extracts has became a great interest in the field of nanotechnology. This green route schemes are eco-friendly, simple, less toxic and environmental benign. In this paper, an environmental friendly approach is presented for the formation of platinum nanoparticles using aqueous leaf extracts of Indian medicinal herbs such as Jatropa gossypifolia and Jatropa glandulifera as an efficient reducing and capping agents. A great transformation pathway of platinum ions to platinum nanoparticles was obtained by employing the Jatropa gossypifolia and Jatropa glandulifera leaves broth with a reaction temperature of 100 °C. Biosynthesized platinum nanoparticles were successfully characterized by Ultraviolet-Visible spectroscopy. FTIR studies confirmed the presence of amine, amide. The -SO 2 functional group were found to be prone for reduction of platinum metal to platinum nanoparticles . Field Emission Scanning electron microscope (SEM) gives information about the shape and size of the formed nanoparticles. Average platinum nanoparticles particle size distribution ranges from nanometer to micrometer. Transmission electron microscope predominantly indicates the biosynthesized platinum nanoparticles that are dodecahedron and some kind of cubic in nature. In addition, biologically synthesized platinum nanoparticles of leaf extracts exhibits a immense potent of antibacterial activity against pathogenic bacteria's.