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A rare case is reported in which a symptomatic Rathke's cleft cyst was studied by light and electron microscopy and tissue culture. The findings are compared with those of a craniopharyngioma studied in the same way. The patient was a 26-year-old woman presenting with headache, chiasmatic syndrome, and hypopituitarism. A cyst containing a mural nodule was partially removed and an Ommaya reservoir placed in the operative site for further treatment. The cyst wall was composed of connective tissue and three kinds of epithelial cells: non-ciliated squamous, ciliated columnar, and mucous-secreting cells. The morphology of these cells in vitro was similar to prickle cells seen in craniopharyngioma and the epidermis. It is concluded that both Rathke's left cyst and craniopharyngioma originate in remnants of Rathke's pouch, but at times may show some histological differences.

HighlightsLaparoscopic subtotal cholecystectomy was successfully performed for a patient.The rates of bile duct injuries is high after laparoscopic cholecystectomy.Removal of the stone is the main purpose of treatment.Laparoscopic subtotal cholecystectomy is useful for patients with Mirizzi syndrome.

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Effect of Creatine depletion by fl-guanidinopropionic acid (/3-GPA) feeding on mitochondrial morphology was studied in rats. Following /3-GPA feeding for 9 weeks, intramitochondrial inclusions running parallel to the long axis of mitochondrion were observed in skeletal muscles. A bundle of inclusions consisted of four parallel arrays containing electron-dense materials. Two outer arrays were continuous with cristal membranes and two inner arrays were chains of fine particles. Mitochondria with such inclusions were elongated probably in only a certain direction even though it was suggested that the layers of cristae were straightened two-dimensionally. Longer mitochondria ran parallel to the muscle fibers. It was also suggested that chronic stimulation of mitochondria) biogenesis by depletion of high energy phosphates may induce such inclusions within mitochondria. inclusions, rat skeletal muscle, j3-It is reported that cold acclimation enlarged the mitochondrial size in rat skeletal muscles (YAHATA and KUROSHIMA, 1977). In these muscles, the mitochondrial cristae and sarcoplasmic reticuli developed better than in the control muscles. Cold acclimation of rats also increased the activity of succinate dehydrogenase in soleus muscle fibers (MATOBA and MURAKAMI, 1981), although the mechanism of morphological and metabolic changes in mitochondria is still unclear. However, our previous study showed that the activity of gastrocnemius oxidative enzymes were elevated when the contents of adenosine triphosphate (ATP) were decreased following the chronic exposure to cold (4°C) In frogs (OHIRA and OHIRA, 1988).