Localization of the mRNAs for fractalkine, a CX Q C chemokine, and for its receptor CX Q CR1 was investigated in the rat brain. In situ hybridization study revealed that fractalkine mRNA was dominantly expressed in neuronal cells particularly in the olfactory bulb, cerebral cortex, hippocampus, caudate putamen and nucleus accumbens. In vitro study using enriched neuronal or glial culture supported the dominant expression of fractalkine mRNA in neurons. On the other hand, CX Q CR1 mRNA was dominantly expressed in glial cells throughout the whole brain. The in vitro study suggested the cells expressing CX Q CR1 mRNA are microglia, not astrocytes or neurons. Fractalkine appears to function as a signal molecule from neuron to microglia.z 1998 Federation of European Biochemical Societies.

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We cloned a cDNA for the rat Ic-opioid receptor from a rat thalamus cDNA library. The deduced amino actd sequence consists of 380 restdues with features shared by members of the G protein-coupled receptor family. The specific bmding of [3H]bremazocine to the membrane of COS-7 cells transfected with the cDNA was displaced by x-specific opioid ligands. but not by p-and S-specific Iigands. Xenopus oocytes injected with the m vitro transcribed mRNA responded to opioid hgands with the same subtype spectficity. Northern blot analysis demonstrated that Kc-optoid receptor mRNA is expressed in a regionally specific manner in rat brain.