Keywords: Antioxidant, calabahs, test antioxidants method with DPPH, free radical. PendahuluanRadikal bebas merupakan suatu atom, gugus atom atau molekul yang memiliki satu atau lebih elektron yang tidak berpasangan pada orbital paling luar, termasuk diantaranya adalah atom hidrogen, logam-logam transisi dan molekul oksigen. Radikal bebas bersifat tidak stabil karena kehilangan elektronnya. Untuk menjadi stabil, radikal bebas akan mengambil elektron dari molekul atau sel lain dalam tubuh kita. Proses pengambilan elektron dari sel-sel tubuh kita menyebabkan kerusakan sel (Holistic, 2011).Berbagai penyakit yang telah diteliti dan diduga kuat berkaitan dengan aktivitas radikal bebas yakni mencakup lebih dari 50 penyakit seperti stroke, asma, pankreatitis, radang usus, penyumbatan kronis pembuluh darah di jantung, penyakit parkinson, sel sickle leukimia, artritis rematoid, pendarahan otak, tekanan darah tinggi dan AIDS (Sari, 2012). Radikal bebas tersebut akan mulai menyerang bagian tubuh yang sehat maupun yang tidak sehat sehingga menimbulkan suatu penyakit. Beberapa penelitian mengungkapkan bahwa senyawa yang dapat menghambat radikal bebas adalah antioksidan. Antioksidan dapat diperoleh secara endogen (enzim SOD atau superoksid dismutase) dan eksogen melalui makanan atau suplemen (Mau, dkk., 2002). Beberapa senyawa antioksidan dapat ditemukan pada tanaman, antara lain berasal dari golongan polifenol, vitamin C, vitamin E, β-karoten dan flavonoid (Hernani & Rahardjo, 2005) Salah satu tanaman yang kaya akan beberapa kandungan fitokimia namun kurang diketahui sebagian besar masyarakat yaitu labu air (Lagenaria siceraria (Molina) Standl.) (Shah, dkk., 2010). Beberapa kandungan fitokimia yang dimaksud adalah kalsium, zat besi, vitamin C, polifenol dan saponin (Robinson, 1995). Menurut Saleh & Marliana (2011),senyawa metabolit sekunder pada ekstrak kasar etanol dari buah labu air (Lagenaria siceraria (Molina) Standl.) adalah saponin, steroid dan fenol. Riset dan Teknologi Kota Bantul menyatakan bahwa daun dan buah Lagenaria leucantha mengandung saponin dan polifenol (Warintek, 2015).
The batik entrepreneurs in Pabean Udik Village are not optimal in developing their businessbecause of problems related to production and marketing. Entrepreneurs do not understand theexact capital cost calculation system and have not made financial reports that are in accordancewith accounting rules. This causes the determined selling price to often experience errors indetermining the cost of goods sold (COGS) which has an impact on the amount of gross profit. Inaddition, batik entrepreneurs do not pay attention to product marketing techniques, and do not evenhave a special concept in packaging their products. Based on the initial visit of the communitydedication team, the fact that the craftsmen only use plastic bags in packaging their products.However, this seems to be due to the fact that batik entrepreneurs have just risen since their businesshas decreased due to the Covid-19 pandemic. So they focus their business capital on product rawmaterials, not for packaging. Entrepreneurs also do not conduct online promotions or sales to invitebuyers. They only market by word of mouth and maintain their customers. With these problems, apersuasive approach and training are needed that are easily understood by batik entrepreneurs withall their limitations so that the services they carry out provide progress for the business managementsystem for batik entrepreneurs in Pabean Udik Village. The method of implementing this serviceconsists of surveys, interviews, and observations. Economic empowerment is carried out by trainingbatik entrepreneurs in Pabean Udik Village regarding basic accounting, introduction of quality raw materials, attractive product design and selling, as well as business digitization with training on theuse of marketplaces. With this training, it is hoped that batik entrepreneurs can apply the knowledgegained and be useful for the implementation of their business operations
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