The purpose of current study was to estimate the incidence of total aflatoxins (B1+B2+G1+G2) in unpack spices and dried fruits. A total of 90 samples included red chillies, black pepper, figs and dried apricots were picked from shops/markets situated in Lahore - Pakistan and were analyzed by using thin layer chromatography (TLC). The results showed that aflatoxin B1 was detected in 24 (26%) samples. The results obtained were ranging between 23.99-97.42 μgkg-1 in spices, 47.68-75.78 μgkg-1 in black pepper, 6.72-14.43 μgkg-1 in figs while 13.2 μgkg-1 of aflatoxin B1 was present in apricot sample. 39.28%, 18.18%, 5.0% and 40% samples of red chilli, black pepper, dried apricots and figs were found contaminated with aflatoxins respectively. Among contaminated samples 32.14% , 13.63%, 5.0% and 15% samples of red chilli, black pepper, dried apricots and figs, respectively were found contaminated with aflatoxins beyond permissible limits. Furthermore, 17.77% i.e. n=16 of the positive samples contained Aflatoxin B1 level more than the permitted limit for entire aflatoxins as lay down by regulatory authority. From the study, it was concluded that a continuous and strict national monitoring plan is needed to improve quality and safety of spices and dried fruits supply in Pakistan.

Dyslexia is a heritable neurodevelopmental disorder characterized by difficulties in reading and writing. In this study, we describe the identification of a set of 17 polymorphisms located across 1.9 Mb region on chromosome 5q31.3, encompassing genes of the PCDHG cluster, TAF7, PCDH1 and ARHGAP26, dominantly inherited with dyslexia in a multi-incident family. Strikingly, the non-risk form of seven variations of the PCDHG cluster, are preponderant in the human lineage, while risk alleles are ancestral and conserved across Neanderthals to non-human primates. Four of these seven ancestral variations (c.460A > C [p.Ile154Leu], c.541G > A [p.Ala181Thr], c.2036G > C [p.Arg679Pro] and c.2059A > G [p.Lys687Glu]) result in amino acid alterations. p.Ile154Leu and p.Ala181Thr are present at EC2: EC3 interacting interface of γA3-PCDH and γA4-PCDH respectively might affect trans-homophilic interaction and hence neuronal connectivity. p.Arg679Pro and p.Lys687Glu are present within the linker region connecting trans-membrane to extracellular domain. Sequence analysis indicated the importance of p.Ile154, p.Arg679 and p.Lys687 in maintaining class specificity. Thus the observed association of PCDHG genes encoding neural adhesion proteins reinforces the hypothesis of aberrant neuronal connectivity in the pathophysiology of dyslexia. Additionally, the striking conservation of the identified variants indicates a role of PCDHG in the evolution of highly specialized cognitive skills critical to reading.

The present study was carried out to investigate the epidemiology and lesions of avian pox in captive peafowl chicks. Overall values of morbidity, mortality and case fatality were 45.2%, 27.1% and 60.0%, respectively. The chicks of 9 to 12 weeks of age showed a significantly (PB0.001) higher prevalence rate than other age groups. The morbidity and mortality due to avian pox in peafowl chicks was significantly (PB0.001) reduced when kept in mosquito-proof cages and hatched under broody chicken hens. Morbidity due to poxvirus infection on the peafowl farm was 82%, 26% and 12% in successive years. This reduction might have been the result of the introduction of mosquito-proof nets after year 1, although this was not the subject of a controlled experiment. All of the peafowl chicks suffering from dry pox showed pustular and nodular lesions on eye lids, beak, legs and toes. Distribution of lesions in different body parts varied significantly (PB0.023). Lesion diameters were less than 1 cm (59.73%), 1 to 2 cm (23.75%) and more than 2 cm (16.87%). Histopathological studies revealed extensive proliferation of subdermal connective tissue and infiltration of heterophils and macrophages. The keratinocytes showed degenerative changes in the form of cytoplasmic vacuolation, ballooning and hyper-chromatic nuclei. Eosinophilic intracytoplasmic inclusions (Bollinger bodies) in keratinocytes were consistently present. It was concluded that avian pox rendered high morbidity, mortality and case fatality in peafowl chicks.

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