Dihydrofuro[2,3-f]indolizidinone obtained from biosourced reagents even at multigram-scale, was used as advanced building-block with up to five points of chemical diversification. This resulted in the sequential synthesis of a series...

A commercial glycosidase mixture obtained from Penicillium multicolor (Aromase H2) was found to comprise a specific diglycosidase activity, β-acuminosidase, alongside undetectable levels of β-apiosidase. The enzyme was tested in the transglycosylation of tyrosol using 4-nitrophenyl β-acuminoside as the diglycosyl donor. The reaction was not chemoselective, providing a mixture of Osmanthuside H and its counterpart regioisomer 4-(2-hydroxyethyl)phenyl β-acuminoside in 58% yield. Aromase H2 is therefore the first commercial β-acuminosidase which is also able to glycosylate phenolic acceptors.

Dihydrofuro[2,3-f]indolizidinone obtained from biosourced L-Glutamic acid (L-GA) was used advantageously as an advanced building-block to prepare optically pure, in few steps affordable spiro[cyclopentane-indolizidine]-tetraol diastereomers as a new oxacarba-spiroindolizidine framework. The...