Latar Belakang: Biji pepaya telah digunakan sebagai antelmintik pada ternak. Teh biji pepaya memiliki aktivitas antiparamphistomum secara in vitro melalui metode adult mortality assay. Karakterisasi teh biji pepaya perlu dilakukan untuk menjamin mutu produk teh yang dihasilkan. Penelitian ini bertujuan untuk menentukan karakteristik fisik dan metabolit sekunder teh biji pepaya (Carica papaya L.). Metode: Kandungan senyawa teh biji pepaya dikarakteristisasi melalui skrining fitokimia. Evaluasi fisik teh biji pepaya meliputi organoleptik, pengukuran pH, penentuan bobot jenis, variasi bobot, dan kadar air. Hasil Penelitian: Teh biji pepaya memiliki rasa pahit, aroma khas, dan berwarna kuning bening, dengan pH 5.61, kadar air 6,876%, bobot jenis 1,000431887 g/ml, dan pada variasi bobo tidak ada bobot yang melebihi 12% limit variasi. Teh biji pepaya mengandung flavonoid dan steroid/triterpenoid. Kesimpulan: Teh biji pepaya memenuhi standar mutu teh. Teh biji pepaya mengandung flavonoid dan steroid/triterpenoid.

Stachytarpheta jamaicensis L. Vahl has traditionally been used as an anti-trematode, but its effectiveness needs to be scientifically tested. This study aimed to determine the anti-trematode activity of the Stachytarpheta leaf extract in vitro. The leaves were extracted using water, and their secondary metabolites were characterized by phytochemical screening. Paramphistomum sp isolated from the rumen was identified by morphometric determination. In vitro analysis of anti-trematode activity was carried out by post-test design, with control groups consisting of positive control (10% Albendazole) and negative control (0.9% NaCl), and the treatment groups consisting of the leaves aqueous extract with various concentrations (2.5%, 5%,10%). The anti-trematode effect was determined by the time required to destroy the worms. The results of phytochemical screening showed that the secondary metabolites contained in the extract were alkaloids, flavonoids, tannins, steroids, and triterpenoids. The average time of worm death was 30 and 240 minutes for positive and negative controls. In comparison, the extract with concentrations of 10%, 5%, and 2.5% killed the Paramphistomum in an average period of 45, 60, and 80 minutes. Amongst the treatment groups, the extract at 10% concentration was the most effective (p<0.05), indicating its potential use as an anti-trematode.