Five horses with lesions compatible with dermatomycosis were selected and skin scrapings were taken from all affected areas. Samples were then treated with 10% KOH and observed by direct microscopic examination; fungal cultures using Mycobiotic Agar and Borelli Agar were also preformed; Trichophyton mentagrophytes was identified in two cases, whereas Candida albicans was identified in the other three. Skin lesions in cases 1 and 2 were treated topically with ketoconazol ointment, while in the remaining cases a weekly bath with propolis-based shampoo and application of propolis-based ointment on the lesions 2 to 3 times a week were implemented. Treatment in all cases lasted 4 weeks. Pictures were taken before and after treatment and, making use of the Image Pro-Express ® software, therapy efficacy was determined by measurement of lesion area diameter and a significant reduction in lesion size was observed by the end of the 4 weeks of treatment with ketoconazole and propolis-based products. Thus proving the effectiveness of such propolis-based products, making them a natural therapeutic alternative for the treatment of cutaneous mycoses in horses, and carrying none of the toxic side effects of conventional pharmaceuticals.