This study was conducted to investigate the effect of swim-up and glass wool as sperm separation techniques with adding vitamin E and superoxide dismutase (SOD) to Tris extender on improving some post-cryopreserved semen quality for Holstein bulls. Low and good of semen were extended using Tris extender. Good semen quality (GSQ) was divided into 3 groups (L1; Tris extender, L2; 2 mM vitamin E, L3; 200 IU SOD) Low semen quality (LSQ) was divided into two main groups, and subdivided into 3 sub-groups (L4; Tris extender, L5; 2 mM vitamin E, L6; 200 IU SOD). In the second main group, swim-up and glass wool techniques were used with adding vitamin E and SOD and subdivided into 3 sub-groups with each technique, and referred to L7, L8 and L9 for swim-up technique and L10, L11 and L12 for glass wool technique. Improving sperms’ cell motility and live sperm and reducing total sperms’ abnormalities percentages of low semen quality were noticed using swim-up and glass wool techniques were used with adding vitamin E and SOD. In conclusion, glass wool filtration separates out dead, abnormal and immotile sperm cells, a good sperm harvested by this technique.
| This study was designed to estimate the biochemical composition of ovary follicular fluid in relation to its size variations in local Iraqi She camels (Camelus dromedarius). Hundred ovary were collected from 50 adult she camel, 4 to 10 years old that slaughtered at Al Najaf abattoir during the breeding season of camelids. The ovaries were transferred immediately to the laboratory in a cold box. Later on, the follicular fluid was collected separately from small and large size follicles, (3-9 mm) and (10-19 mm) respectively. The fluid was kept at -4 0 C for further analysis. The follicular fluid samples were analyzed to estimate the metabolic composition (cholesterol, glucose and total protein), and the ionic compositions (calcium, sodium and potassium). The results of this study revealed significant (P< 0.05) increase inthe glucose and cholesterol concentration, while there were significant decrease in the total protein in large size follicles. Meanwhile, significant increase was seen in the concentration of Na+ and Ca+2 in relation to the size of the follicles. However, significant decrease was occurred in the concentrations of K with increasing follicle size. In conclusion, the results of this study revealed a significant variations in the concentration of the follicular fluid metabolic and the ionic compositions with the variations of its size.
The study was conducted on (50) Friesian cows age (2.5 -5) years with different nutritional levels, suffering from dysfunction ovary for the period (40-55) days after birth of the year (2019). In the milk cows station (Faiha) / Babylon province. To treat the cases and then follow back to the estrus and use artificial insemination with follow-up pregnancy. The study relied on dietary and hormonal therapy during the open period of adult (110-125) days, cows were divided into two groups (non-functional ovaries and control), which consecutively was divided into three sub groups, including control and treatments. hormone therapy must be linked to poor food. Weaknesses or functional delays are not recommended to used hormonal therapy unless the animal’s health and nutritional status are improved, As well as the genetic status of the animal dependent on breed selection in terms of nutritional conversion and access to sexual puberty and its ability to maintain the balance of energy is the most important thing that makes the animal in the normal situation in terms of production during his life. Progesterone and GnRH therapy also play a role in the reactivation of ovaries in cases of delayed and hostile. Functional delays are also easier to treat, which is done by dietary improvement only and may not need hormone therapy, especially after adjusting the nutritional status in particular. We conclude from this study that the nutrition improvement before and after birth and improve the quality is the most important things in the management and breeding of animals and during their life cycle before puberty and focus on the postpartum period to restore the animal to its normal functional activity.
The present study was undertaken to observe the effect of the aqueous leaf extract of Aloe vera gel of the reduction in blood glucose and lipid level in hyperglycemia and hyperlipidemia rats. Twenty four male rats were divided into four groups (6 rats/group) and treated as follows for 28 days.G 1 were intubated orally distal water serving as control while, G2 were administrating of Dexamethason -induced diabetic 1 mg/kg/bw. Intraperitoneally, G3 were administrating of diabetic rats given Dexamethason 1mg/kg/bw. + Aloe vera leaf gel extract (300 mg/kg) using an intragastric tube, G4) were administrating of diabetic rats given Dexamethasone 1 mg/kg/bw. intraperitoneally + Aloe vera leaf gel extract (500 mg/kg) using an intragastric tube, the following criteria were measured sugar levels and blood lipid profile. The findings show that Aloe Vera had a significant decrease influence in serum glucose, cholesterol, TG, and VLDL and a significant decrease HDL. In conclusion, this study mention a new evidence of the role of Aloe Vera on the decreasing glucose, lipid profile.
Hepatotoxicity is a common clinical manifestation associated with a wide range of anticancer therapies. Because of the inherent toxicity of anticancer therapies, oncologists must maintain a broad understanding of their effects on the body, including the liver.Therefore, the study was conducted to examine the effects of two doses of L-carnitine on damaged liver as a result of giving cytarabine. Twenty-four male rat were used in the experiment. The rats divided to four group each group with six rats,the first group injected normal salineintraperitoneally and set as control group(G1),the second group injected cytarabine 25 mg/kg/bw intraperitoneally and set as (G2), the third injected cytarabine 25 mg/kg/bw and 50 mg /kg/bw L-carnitine intraperitoneally and set as (G3), the third injected cytarabine 25 mg/kg/bw and 300mg/kg/bw L-carnitine Intraperitoneally and set as(G4), the study showed that use of L-carnitine reduced the harmful effect of cytarabinein the body when compared to the group that was injected only with cytarabineat level of (p-value <0.05). ALT show a significant increase in G2 when compared to other groups the mean value was (104±7.697, 134.66±13.08, 105.66±5.89, 94.33±3.5) for groups: control, cytarabine,50mg/kg/bw & 300mg/kg/bw ofL-carnitine respectively;,,
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