The present study was carried out to evaluate the effects of moringa leaf powder through dietary supplementation in guppy fish (Poecilia reticulata) on skin immune responses and growth performance. A total number of 240 fish (average body weight of 0.13 ± 0.01 g) were equally divided into four feeding groups including 0% (T0), 5% (MT1), 10% (MT2) and 15% (MT3) concentration of moringa leaf powder. The results showed that levels of lysozyme activity and alternative complement activity (ACH50) significantly increased with increase the moringa leaf powder (p < .05) concentration. Regarding the myeloperoxidase activity results, 15% doses of moringa leaf powder showed the highest amount of myeloperoxidase activity as compared to control group (p < .05). Total immunoglobulin content was increased significantly 10% and 15% treatments as well. In the present investigation, dietary supplementation of Moringa oleifera leaf powder had no significant impact on weight gain, net weight gain, specific growth rate, FCR and survival rate. The present results concluded that a dietary dose of 15% moringa leaf powder per kg of feed can significantly improve skin mucus immunity through enhanced production of mucus in the guppy.

The present study was carried out to evaluate the effects of replacing fish meal with dietary Arthrospira platensis as a feed supplementation on growth performance, survival and skin pigmentation of guppy, Poecilia reticulata. A total number of two hundred forty healthy fishes (average body weight 0.15±0.02) were equally divided into four feeding groups (triplicates) as such as control (T0), 5% (ST1), 10% (ST2) and 15% (ST3). The final experiment was conducted in aquarium for 60 days. The average crude protein and Crude lipid levels of the diet is 10.31 & 39.81%, respectively. A significant increase in the concentration of carotenoids in fish skin was observed with an increase in spirulina concentration in fish feed. However, the ST3 treatment had highest carotenoids concentration. Redness, yellowness and whiteness found to have significant differences. There was a significant difference between treatment groups concerning the growth parameters. The ST3 diet significantly increases length, weight, net weight gain, weight gain, specific growth rate and survival rate. Inclusion of 15% Spirulina was found to be suitable to ensure growth as well as pigmentation of guppy. Spirulina powder can be used as protein and carotenoid supplements in fish feed to improve growth, survival and colouration of guppy.