Traditionally medicinal plants are used for its potential chemotherapeutic action and for safety upon the continued use of these plants. Achillea millefolium (AM) is an ancient herb which is used to treat wounds, headaches, disorder in gastrointestinal and hepatobiliary, pain, and inflammation. This study was aimed to investigate the biochemical parameter of antimutagenic methanolic extract for AM in vivo. Plant extract together with MTX decreased the damage caused by the drug on the concentrations of total cholesterol and triglycerides (160.3±2.26 and 108.7±2.71), significant increase in total protein concentration (8.51±0.17, 9.46±0.19 and 10.8±2.71 g\dL) for 100 and 200 mg\kg was observed. Also plant extracts counteract the damage caused by drug through the reduction for both creatinin and albumin concentrations to 1.57±0.03 mg\dL and 4.56±0.09 g\dL, respectively. On enzymatic level, the results of interactions indicated the ability of plant to modulating harmful effects of the drug (57.1±1.3, 130±3.1 and 111.1±2.1 U\L) for GOT, GPT and ALP, respectively. On the other hand, the plant possesses the ability to enhance the concentrations of all immunoglobulin (IgM, IgA and IgG) in the two doses tested. Therefore, the results of this study indicate the antimutagenic potential of AM extract and encouraging its consumption with MTX as one of the promising therapies.

The objective of this study was to considered as an explorer for In vivo studies on the production of some secondary metabolites from local medical plants named Cinnamomum zeylanicum to study their effect on mice organs that treated with Cladosporium sp. extract (In vivo). Preparation of water extract of Cinnamomum zeylanicum. different doses were prepared (20,40,60,80%) of plant which (9) male mice were used and divided into three control group and dosed with distilled water mice were administrated with first dose 1ml/kg of Cladosporium extract for two weeks and mice were administrated Cladosporium with 80% of the aqueous extract of Cinnamomum zeylanicum at a dose of 1 ml / kg daily for two weeks. Mice treated with Cladosporium sp. fungal filtrate caused vascular congestion in the tissues of the liver resulting in cell bleeding, in addition to many changes in chromotin in and nuclei sized increased while the results indicate the ability of the mixture between fungal filtrate and Cinnamomum to counteract these adverse effect in mice and return its appearance looks like normal also the kidney of animals treated with Cladosporium fungal filtrate caused retention of congested glomeruli with a large pool of fluids while the result of interaction caused inhibition in radial growth of the fungus and reduced its effectiveness on kidney function and the synthetic form of the tissue.

Rhizomes of Zingiber officinale Roscoe. belonging to the family Zingiberaceae is used for bronchitis, as a carminative, for treating cough, cataracts and as a stimulant. The rhizome is cooked with salt and water and used as an expectorant. Extract of the juice of rhizome is used as eye-drops. In Yemen, it is mixed with other plants used for constipation, as a purgative, against colds, catarrh and acidity of the stomach. Root ginger is widely used for culinary purposes and as a spice. The rhizomes (imported from India) are used with cinnamon and cloves and made into a tea for treating colds and as a general tonic. The drink is also used as an aphrodisiac (Gazanfar, 1994). Ginger is useful in piles, rheumatism, headache, lumbago, pains, bleeding, chest congestion, cholera, cold, diarrhea, dropsy, nausea, stomachache, gastrointestinal disorders, vomiting, and diarrhea. The fresh juice of ginger acts as a strong diuretic. The juice of the leaves is effective against helminthiasis and marasmus and related conditions of diarrhea and dysentery (Monograph of Unani Medicine,2003).