A chiropteran inventory of Himachal Pradesh, northwestern India is presented. Based on field observation and the study of museum collections and published literature, the occurrence of 28 species within 14 genera and five families is affirmed. The study also provides observations on ecology and biology of selected species, and ecological, zoogeographical and conservation aspects of the chiropteran fauna of Himachal Pradesh are also discussed. Considering the lack of studies on the bat fauna of Himachal Pradesh, it is expected that systematic and intensive field surveys will refine significantly our knowledge of diversity and distribution of Chiroptera in the state.