Aim To determine and compare antifungal activity of two tissue conditioners combined with nystatin, miconazole and fluconazole against Candida albicans. Materials and methods Two tissue conditioners Viscogel and GC Soft combined with nystatin, miconazole and fluconazole were tested against Candida albicans using agar core inhibition diameter assay. One-way analysis of variance followed by Tukey's post-hoc test was used to test the intergroup difference. p-value < 0.05 was considered statistically significant. Results Maximum inhibition was seen in the fluconazole groups followed by miconazole and the least inhibition was seen in case of nystatin group. Conclusion Tissue conditioners when mixed with antifungal agents showed satisfactory inhibition of Candida albicans. Clinical significance Incorporation of antifungal drugs into tissue conditioners shows good inhibition of C. albicans and can be recommended for clinical use. How to cite this article Chopde N, Pharande A, Khade MN, Khadtare YR, Shah SS, Apratim A. In vitro Antifungal Activity of Two Tissue Conditioners Combined with Nystatin, Miconazole and Fluconazole against Candida albicans. J Contemp Dent Pract 2012;13(5):695-698.