One of the viral diseases affecting both domestic and wild animals in many countries around the world is foot and mouth disease (FMD). The livestock sector has been suffering significant financial losses for many years as a result of the disease. The present research work was focused on the isolation, documentation and serotyping of the Foot and Mouth Disease (FMD) virus from different locations. Reverse Transcription Polymerase Chain Reaction (RT-PCR) was used to detect the FMD virus (FMDV) serotype-O that is currently circulating in the BHK-21 cell line. All the tested samples were positive for FMDV and specific for serotype-O was 64.67-52.67% from tongue epithelium tissues and 30.67-16.67% from foot tissues respectively based on locations. In BHK-21 cell lines, the viruses caused cytopathic effects such as rounding, enlargement, intercellular bridge breakdown, and finally, death of cells, which shows the presence of the FMD virus under an inverted microscope. The BHK-21 cell culture fluid was found positive for FMDV in cell culture and also was positive based on CPE. Once more, the resulting inoculum was used to serotype the virus using RT-PCR. Based on the result of the RT-PCR method, the conclusion reached was that the serotype of the FMD virus was ‘Type O’. Asian J. Med. Biol. Res. 2023, 9 (2), 14-22

Cloud service broker (CSB) as an emerging technology intermediates heterogeneous multiple cloud services for both the providers and consumers. Recently, Cloud computing & mobile cloud computing applications (MCA) have gained an enormous popularity, which has led to an increasing need for the development of platform independent Middleware/CSB to support all types of cloud service consumer applications including x86*x64 based standard OS & ARM based mobile applications, web browsers, etc. Developing Platform Independent Hybrid CSB, however, is not an easy task. Developers have to deal with difficulties inherent from the different cloud controllers, cloud service providers environments, clients’ application types, network connection types (wired, wireless), GPS (Global Positioning Systems) information of cloud resources and clients’ etc. In this thesis, the proposed design of a middleware/CSB that abstracts the real-time resources of various clouds (private, public, home, Local) and stores the resources in its own Database. It will also store clients requests then analyzes the request to find the nearest available servers which is running the appropriate applications. Then the CSB will forward the destination servers information to the clients. Thesis goal is to achieve context awareness, location awareness, platform independence, portability, efficiency, and usability. Portability is achieved by following the J2ME platform specifications. The middleware has been implemented and tested on a real time Openstack cloud using by our newly designed Android Clients and platform independent Mozilla Firefox browser. The performance measurements of the middleware show that it achieves its efficiency requirements. Furthermore, the middleware’s database can be used for resource algorithm, pattern analysis, and for future requirements.