Throughout this study, the objective was to determine the most effective carbohydrate (CHO) sources under different light-emitting diodes (LEDs), and the impact of chlorocholine chloride (CCC), for the in vitro regeneration of the protocom-like bodies (PLBs) in Phalaenopsis ‘Fmk02010’. We applied 15 LEDs combined with three CHO sources and five CCC concentrations in the study. Organogenesis of PLBs was very poor in maltose both for the number of PLBs and their fresh weight (FW) compared to media containing sucrose and trehalose. Sucrose was the best CHO source under the red-white (RW) LED for the in vitro organogenesis of PLBs (PLBs: 54.13; FW: 0.109 g), while trehalose was best under the blue-white (BW) LED (PLBs: 36.33, FW: 0.129 g). The red-blue-white (RBW)-trehalose combination generated a suitable number of PLBs (35.13) with the highest FW (0.167 g). CCC at 0.01, 0.1, and 1 mgL−1CCC had no effect on PLB formation or FW, but 10 mg L−1 reduced both. RW-sucrose, BW-trehalose, and RBW-trehalose were the best combinations for PLB organogenesis. The addition of low concentrations of CCC in the plant culture medium are unnecessary.

The objective of this study was to identify effective carbon sources for the in vitro propagation of PLBs in Dendrobium cultivars Dendrobium kingianum ‘Hallelujah’ and Dendrobium k. Jonathan’s Glory ‘Dark Joy’. In this study, we used two types of carbon sources to culture the cultivars under five different LED lights. For, both carbon sources the highest numbers of PLBs were obtained with the green LEDs compared with the other LED lights. For Dendrobium kingianum ‘Hallelujah’ the trehalose supplemented medium produced the highest number of PLBs (13.8/explant) and the maximum fresh weight (0.45g/explant) under green LEDs. On the other hand, for Dendrobium k. Jonathan’s Glory ‘Dark Joy’, the sucrose supplemented medium produced the highest number of PLBs under green LED (8.0/explant, fresh weight 0.22g/explants) and the trehalose supplemented medium produced the maximum number of PLBs under white LEDs (8.1/explants, fresh weight 0.23g/explants). The sucrose was the most relevant carbon source for the in vitro organogenesis of Dendrobium k. Jonathan’s Glory ‘Dark Joy’, while trehalose was best under white LEDs.

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