Assessing fish habitat suitability in a data-scarce tidal river is often challenging due to the absence of continuous water quantity and quality records. This study is comprised of an intensive field study on a 42 km reach which recorded bathymetry and physical water quality parameters (pH, electroconductivity, dissolved oxygen, and total dissolved solids) testing and corresponding water levels and velocity. Frequent water sampling was carried out on 17 out of 90 locations for laboratory water quality tests. Based on this, an interpolation technique, i.e., Inverse Distance Weighted (IDW), generates a map in a Geographic Information System (GIS) environment using ArcGIS software to determine the river water quality parameters. Additionally, a hydrodynamic model study was conducted to simulate hydraulic parameters using Delft3D software followed by a water quality distribution. During validation, the Delft3D-simulated water quality could reasonably mimic most field data, and GIS featured dissolved oxygen. The overall water quality distribution showed a lower dissolved oxygen level (~3 mg/l) in the industrial zone compared to the other two zones during the study period. On the other hand, these validated hydraulic properties were applied in the Physical Habitat Simulation Model (PHABSIM) set up to conduct the hydraulic habitat suitability for Labeo rohita (Rohu fish). Thus, the validated model could represent the details of habitat suitability in the studied river for future decision support systems, and this study envisaged applying it to other similar rivers.