Favipiravir is a synthetic prodrug, which was first discovered while assessing the antiviral activity of chemical agents active against the influenza virus in the chemical library of Toyoma chemicals. It works by inhibiting RNA dependant RNA polymerase (RdRP), an enzyme required for RNA viral replication inside human cells. A simple, rapid, and economic method was developed for the quantitative determination of Favipiravirusing spectrofluorometer. The Favipiravir standard drug solution and sample tablet solution was prepared using double distilled water as a diluent. The different concentrations ofpure drugin the range 2-10 μg/ml and one sample solution were measured for the intensity at 432nm in the spectrofluorometer. The calibration curve was plotted and the sample’s unknown concentration was calculated from the plot. The calibration curve was found to be linear with r2 value obtained as 0.99.There are various other methods available for the quantification of Favipiravir which include RP-HPLC, UV-spectroscopic methods, FTIR, LC-MS with different extraction methods spiked in human plasma but not by using spectrofluorometer. Favipiravir shows fluorescence when dissolved in appropriate solvent hencethis method was developed to quantify Favipiravir which is a simple and efficient method. This method developed is easy and can be used for routine quality control test for Favipiravir pharmaceutical formulations. Keywords: Favipiravir, spectrofluorometer, Calibration curve.
Two simple, easy, and rapid methods were developed for the determination of glucose reducing sugar in various soft drink samples and total reducing sugars in honey sample. A colorimetric method was developed to analyze the soft drink samples (non-diet and not dark colored) and titrimetric method was developed to determine the total reducing sugars in honey sample. The various soft drink samples obtained from market were analyzed for the glucose content in them using UV-Visible spectrophotometry and DNSA solution. The DNSA imparts color to the sucrose standard and samples and later the absorbance values were checked at 580 nm. The calibration curve of the standard sucrose solution was plotted and the concentration of the samples were determined using the calibration curve. The titrimetric method is based on the principle of Lane Eynon method for determination of reducing sugars and it involves two steps: standardization of invert sugar and titration of honey solution using Fehling’s A and B solutions. There were no titrimetric methods available in literature for determination of total reducing sugars with use of simple reagents. Hence the proposed methods were developed which are rapid, and reliable.
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