Biohydrogen production is most important for simultaneous energy generation. Hydrogen (H2) is considered as a suitable substitute source of energy because of its regenerative, carbon neutral and high energy yielding. However, to optimize key factors affecting hydrogen production from water hyacinth by heat treated anaerobic fermentation process. Biological methods is a potential option to meet the growing clean energy needs for hydrogen production. This paper was discussed about key factors affecting namely substrate concentration.
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