A total of 32 seed samples collected from 11 locations were tested for associated seed borne mycoflora by using blotter method and Agar plate method. In blotter method, eight mycoflora were identified based on spore morphology namely, Alternaria alternata, Aspergillus flavus, Aspergillus niger, Curvularia lunata, Fusarium sp., Bipolaris oryzae, Penicillium sp. and Trichoconis padwickii. Most frequently occurring fungi genera were Bipolaris (25.76%), followed by Fusarium (20.92%) and least in case of Penicillium (3.33%). While in agar plate (PDA), maximum of 10 seed mycoflora like A. alternata, A. flavus, A. niger, C. lunata, Fusarium sp., Bipolaris sp., Nigrospora sp., Pencillium sp., T. padwickii and Trichoderma sp. were observed. Maximum percent occurrence was observed in genus Fusarium (22.67%), Curvularia (20.06%) and least in Nigrospora (0.15%) and Trichoconis (0.15%) respectively. Agar plate method showed maximum number of mycoflora on seeds than blotter method. However, it was observed that Bipolaris was predominant in blotter method and Fusarium in agar plate method.
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