Problem statement and aim The esthetics of the complete denture primarily depend upon the color of the denture teeth; however, there are situations where the teeth are subjected to extrinsic and intrinsic stains and discolor over time. The purpose of the study was to investigate the effects of smoking and two different denture cleaners on the color stability of the denture teeth. Material and methods Commercially available maxillary anterior teeth made up of acrylic resin were selected for the study and were divided into two groups (n=10): persulfate-free denture cleanser and persulfate containing denture cleansers. The acrylic teeth were set in the smoke chamber with a distance to absorb the smoke equally from the cigarette. The smoke was released for 10 minutes, and the results are observed by the spectrophotometer. Results All the values were collected after the 21 st day, and data were analyzed with the SPSS software. It was found that denture cleansers with persulfate are effective on color stability. Conclusions Even though the persulfate containing denture cleansers are injurious to health, they can be recommended to the smokers with clear instructions of use. However, for non-smokers, persulfate-free denture cleansers are preferred over the persulfate containing denture cleansers.