The study investigated the efficacy of two natural, plant-derived antimicrobials (PDAs), namely trans-cinnamaldehyde (TC), and eugenol (EG) for decreasing Acinetobacter baumannii adhesion to and invasion of human keratinocytes (HEK001). Moreover, the efficacy of two PDAs for inhibiting A. baumannii biofilm formation was determined using an in vitro collagen matrix wound model. Additionally, the effect of TC and EG on A. baumannii biofilm architecture was visualized using confocal scanning microscopy. Further the effect of both PDAs on genes critical for biofilm synthesis was determined using real-time quantitative polymerase chain reaction. Both TC and EG significantly reduced A. baumannii adhesion and invasion to HEK001 by ~2 to 3 log CFU/mL (p < 0.05) compared with the controls (p < 0.05). Further, after 24 and 48 h, TC and EG inhibited biofilm formation by ~1.5 to 2 and ~2 to 3.5 log CFU/mL, compared with controls (p < 0.05). Confocal microscopy revealed that TC and EG disrupted the biofilm architecture. RT-qPCR results indicated that two phytochemicals significantly down-regulated the transcription of genes associated with A. baumannii biofilm production. The results suggest that both TC and EG could potentially be used to treat A. baumannii wound infections; however, their efficacy in in vivo models needs to be validated. Copyright © 2016 John Wiley & Sons, Ltd.

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Se reduced extracellular and intracellular verotoxin concentration by 40-60% and 80-90%, respectively (p < 0.05), and downregulated verotoxin genes (p < 0.05). Se reduced Gb3 receptor synthesis in lymphoma cells, and real-time quantitative PCR data revealed a significant downregulation of LacCer synthase gene (GalT2) involved in Gb3 synthesis. Further studies are warranted to validate these results in an appropriate animal model.