No abstract

Perhaps, in small children or when using casts in bigger children, a bioabsorbable suture may be used for fracture stabilization avoiding the need for extensive surgery to remove the fixation material.

No abstract

Co‐expression of proteins in bacteria offers an important strategy for producing multiprotein complexes for biochemical and biophysical studies. We have found that co‐expression of histones H2A and H2B (from yeast, chicken or Drosophila) leads to production of soluble heterodimeric H2AH2B complexes. Drosophila histones H3 and H4 can also be produced as a soluble (H3H4)2 heterotetrameric complex if they are co‐expressed with the histone chaperone Asf1. The soluble H2AH2B and (H3H4)2 can be purified by simple chromatographic techniques and have similar properties to endogenous histones. Our methods should facilitate histone production for studies of chromatin structure and regulatory proteins that interact with histones. We describe a simple strategy for constructing co‐expression plasmids, based on the T7 RNA polymerase system, which is applicable to other systems. It offers several advantages for quickly creating plasmids to express two or more proteins and for testing different combinations of proteins for optimal complex production, solubility or activity.

The protective effects of botulinum toxin A against flap necrosis after perforator twisting and its underlying molecular mechanism in a rat model.