There is little information on human Q fever in Iran and other Middle East countries. The aim of this study was to determine apparent Q fever seropositivity among febrile patients with suspected brucellosis in southeast Iran. Coxiella burnetii phases I and II specific IgG antibodies were measured by a commercial enzyme-linked immunosorbent assay (ELISA) in sera from 75 febrile patients. Phase I antibodies were detected in 18 subjects (24%) and phase II antibodies in 27 subjects (36%). This is the first report of human Q fever seropositivity in Iran after 3 decades and demonstrated a high prevalence of C. burnetii exposure in the sampled febrile patients.

Clostridium perfringens type D infects ruminants and causes the enterotoxemia disease by ε-toxin. A mutated ε-toxin gene lacking toxicity was designed, synthesized, and cloned into the pT1NX vector and electroporated into Lactobacillus casei competent cells to yield LC-pT1NX-ε recombinant strain. BALB/c mice, immunized orally with this strain, highly induced mucosal, humoral, and cell-mediated immune responses and developed a protection against 200 MLD/ml of the activated ε-toxin. This study showed that the LC-pT1NX-ε could be a promising vaccine candidate against the enterotoxemia disease.

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Background: There are several protocols to extract DNA from Lactobacillus spp. In the case of L. casei it is harder because of its especial and thick cell wall. Objectives: In this study, nine DNA extraction protocols (by lysozyme treatment) were evaluated and compared in two categories (traditional and kit-based protocols) and an improved method was presented. Materials and Methods: DNA quantity and quality was determined by spectrophotometry, agarose gel electrophoresis and polymerase chain reaction (PCR). Results:The results revealed that the yield of extracted DNA differed by each protocol (5.8 -17.1 μg/100 μL), but provided appropriate DNA for PCR amplification. The modified protocol offered the best total DNA extraction method when both quality (DNA purity; 1.54 μg) and quantity (DNA yield; 17.1 μg) were considered. Conclusions: We suggest this protocol for effective and inexpensive DNA isolation from L. casei for downstream biological processes such as PCR.