Aim: This study aimed to evaluate the relationship between collaboration between nurses and patient safety culture. Method: This cross-sectional study was conducted with 411 nurses working in a public hospital between February and May 2018. Data were collected with the descriptive characteristic form, the Nurse-Nurse Collaboration Scale, and the Patient Safety Culture Scale. Independent sample t-test, one-way ANOVA and correlation analysis were used to analyze the data. Results: Collaboration among nurses and patient safety culture were found to be moderate. The correlation coefficient between collaboration and patient safety culture was 0.523, indicating a positive relationship. Conclusion:The relationship between the collaboration between nurses and patient safety culture were found to be moderate. As the collaboration between nurses increases, the patient safety culture of nurses also increases. In this context, nurse managers should plan activities to increase collaboration among nurses and ensure that nurses receive more training to improve the patient safety culture.