Transferrin (Tf) has been effectively used to promote the cellular uptake of HAIYPRH (T7) peptide conjugated nano-drugs. Herein, the enhancing effect of Tf on T7 decorated nano-drugs transport is investigated...
Folic acid (FA) is a ligand that has been renowned for
its strong
binding to FA receptor (FR), and the robustness of the specific interaction
has led to the generation of multitudinous tumor-targeted nano-drug
delivery systems. However, selecting the appropriate FA targeted nano-drugs
according to types of cancerous cells to achieve a high effect is
critical. Understanding of how the drug is transported through the
cell membrane and is delivered intracellularly is very important in
screening ideal targeted nano-drugs for cancerous changes in different
organs. Herein, by using a force tracing technique based on atomic
force microscopy (AFM), the dynamic process of FA-polyamidoamine-Doxorubicin
(FA-PAMAM-DOX) entry into different tumor cells (HeLa and A549) and
normal cells (Vero) was monitored in real time. The cell membrane
transport efficacy of FA-PAMAM-DOX in tumor cells with an FR high
overexpression level (HeLa) and FR low overexpression level (A549)
is analyzed, which is significantly higher than that in normal cells
(Vero), especially for HeLa cells. Subsequently, the intracellular
delivery efficiency of FA-PAMAM-DOX in different cell lines was measured
by using fluorescence imaging and AFM-based nanoindentation techniques.
This report will help to discover the cellular transport mechanism
of nano-drugs and screen out optimal therapeutic nano-drugs for different
types of tumors.
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