In Ethiopia, Oxytenanthera abyssinica A.Rich. Munro has varies economic importance. However, conventional propagation methods of O. abyssinica are generally inefficient due to their low multiplication rate, time consuming, labor intensive, and too costly. The objective of this study was to develop a protocol for mass micropropagation of O. abyssinica through seed culture. Murashige and Skoog (MS) medium augmented with 6-Benzylaminopurine (BAP) was used for shoot initiation and multiplication. For in vitro rooting, MS medium supplemented with 3-Indole –butric acid (IBA) was used. In shoot initiation experiment all viable seeds were proliferated in 5-7 days of culturing. In shoot multiplication at 0.004 g/L BAP was Sucssefuly shoot multiplied, also best root responding were found at 0.005 g/l IBA. The present optimized protocol enables for any acters who needs large numbers of low land bamboo seedling for industery, small and micro enterprize or for reafforestation programms.

The African Sandalwood plant (Osyris lanceolata) is a threatened shrub or a small hemiparasitic tree endemic to East Africa and South African regions, which is being severely affected by fungus, uproot-ing for oil extraction, poor natural regeneration, phenological structures (dioecious), medicinal values, lack of sexual recruitment, habitat loss, anthropogenic and climate factors and life span of its seed is short. It has been found a chllange through application of in situ conservation of natural trees like osyris lanceoleta since rapid human population growth and dramatically change the demands of fuel wood and agriculture investement, the available natural strands of valuable plants of African sandalwood have not been able to meet the demands of the people in world specifically developing countries. How ever, this study via using the advances of plant propagation method it provide new options for conserving and multiplication of Osyris lanceolata species using in vitro culture techniques of using IBA hormone by taking plant material of the targated plant .Propagation of African sandalwood (Osyris lanceolata) by rooting hormone of IBA in non-mist poly-propagator was investigated by taking young stem of osyris lanceoleta from Bazawit Hill and we provide an alternative propagation technique to the use of seeds. This Hill is near to Bahrdar town , Ethiopia.New leaf were initiated on the young stems of the osyris after six weeks of the experment.The influence of IBA as rooting promoter at three concentrations (50, 100 and 150 ppm) were recorded.Un expectedly,from the data collected it was observed that the success 83.3% can be achieved from young stem of un treatead cuting gave a new leaf and 50% were achived in the treatead plant, this propagation technique is a viable alternative to seed. The success may influenced by application orgin of a stem cutting with a type of soil.

In the recent years medicinal and other economical important plants are getting attention due to the presence of therapeutically important active contents; however over exploitation and propagation problems are the major concern for conservation of several econmically important plant species.Among economical imprortant plants an attempt has been made to develop a propagation method for African sandalwood (Osyris lanceolata) by air layering approach aiming at providing an alternative propagation technique to the use of seeds or cuttings that germinate or root poorly. Air layers were applied root hormone to the stem branches of Osyris lanceolata (still attached to the plant) during Novmbere 2019, at Bazawit Hill,Nothern Ethiopia at edge of Blue-Nile River from its source at Lake Tana .Root initiation were starts after 12 weeks of the experments.The factors assessed in this experiment were the effect IBA as rooting promoter at three concentrations (0,50, 100 and 150 ppm).From the data collected it was observed 88.8% rooting were achieved from air layers in the mother plants it selef those treated by IBA hormone and the controls not responding root.Making this propagation technique is a viable alternative to the use of seed or cutting propagation. Rooting response success were influenced by application of rooting hormone of IBA,soil composition and the seasons . At a rate of 150 ppm 100% all expermented plants were intiated and primarly other than other treatmeants responded root. The significance enhancing of root making on Osyris lanceolata plants stem via air layers is linked to the advantage of more rooting hormone concentartion.