In this study, the antioxidant activities, total polyphenolic contents of algal extracts from Gongolaria barbata were examined by using ethyl acetate (AcOEt), water(W), and phosphate buffer (PH) as solvents. According to the results, EtOH, W, and PB showed medium antioxidant potential with low IC50 values. The highest total flavonoid content was detected in W (6.91±0.09 mg/g Ext., and PB showed the maximum phenolic content (2.29±0.01 mg GAE/g ext.). The chlorophyll a and total carotenoid contents were detected as 40.5±1.20 and 29.13±1.12 (mg/g DW), respectively. Phycoerythrin was 0.002±0.001 (mg/g DW), while phycocyanin content was found as0.04 ±0.01 (mg/g DW). Our results show that PB could be used as an extraction solvent for determining the antioxidant properties of G. barbata instead of ethanol.