The livestock species economically enhance the quality of life of farmers and industries and reproduction is the only way to progress in the next generation. Kisspeptin (Kp) is a neuropeptide that acts on the reproductive system and is associated with reproductive disorders in mammalians. The different forms of Kp are present but only kp-14 are more prominent to use due to their short length. The Kp is act with the orphan G-protein coupled receptor 54 (GPR54) ligands and activates the hypothalamic-pituitary-gonadal axis (HPG) to release GnRH and reproductive hormones. Even the Kp is a reproductive neuropeptide, it's limited to a few species to conduct the research and needs to perform more research on various species. The Kp may act powerful supplement in animal reproductive techniques and this review focuses on the livestock and pet animal reproductive system regulated by Kp.