Sfmbt2-hosted miR-466a-3p and its close relatives are often among the most significantly up-regulated or down-regulated miRNAs in responses to numerous deleterious environmental stimuli. The exact roles of these miRNAs in cellular stress responses, however, are not clear. Here we showed that many Sfmbt2-hosted miRNAs were highly hypertonic stress responsive in vitro and in vivo. In renal medulla, water deprivation induced alterations in the expression of miR-466(a/b/c/e/p)-3p in a pattern similar to that of miR-200b-3p, a known regulator of osmoresponsive transcription factor Nfat5. Remarkably, exposure of mIMCD3 cells to an arginine vasopressin analog time-dependently down-regulated the expression of miR-466(a/b/c/e/p)-3p and miR-200b-3p, which provides a novel regulatory mechanism for these osmoresponsive miRNAs. In cultured mIMCD3 cells we further demonstrated that miR-466a-3p and miR-466g were capable of targeting Nfat5 by interacting with its 3'UTR. In transgenic mice overexpressing miR-466a-3p, significant down-regulation of Nfat5 and many other osmoregulation-related genes was observed in both the renal cortex and medulla. Moreover, sustained transgenic over-expression of miR-466a-3p was found to be associated with polydipsia, polyuria and disturbed ion homeostasis and kidney morphology. Since the mature sequence of miR-466a-3p is completely equivalent to that of miR-466e-3p and that the seed sequence of miR-466a-3p is completely equivalent to that of miR-297(a/b/c)-3p, miR-466d-3p, miR-467g and miR-669d-3p, and that miR-466a-3p differs from miR-466(b/c/p)-3p only in a 5' nucleotide, we propose that miR-466a-3p and many of its close relatives are important epigenetic regulators of renal Nfat5 signaling, osmoregulation and urine concentration in mice.

A promising approach for the development of effective full-color displays is to combine blue microLEDs (μLEDs) with color conversion layers. Perovskite nanocrystals (PNCs) are notable for their tolerance to defects and provide excellent photoluminescence quantum yields and high color purity compared to metal chalcogenide quantum dots. The stability of PNCs in ambient conditions and under exposure to blue light can be improved using a SiO 2 coating. This study proposes a device that could be used for both display and visible light communication (VLC) applications. The semipolar blue μLED array fabricated in this study shows a negligible wavelength shift, indicating a significant reduction in the quantum confined Stark effect. Owing to its shorter carrier lifetime, the semipolar μLED array exhibits an impressive peak 3 dB bandwidth of 655 MHz and a data transmission rate of 1.2 Gb/s corresponding to an injection current of 200 mA. The PNC–μLED device assembled from a semipolar μLED array with PNCs demonstrates high color stability and wide color-gamut features, achieving 127.23% and 95.00% of the National Television Standards Committee standard and Rec. 2020 on the CIE 1931 color diagram, respectively. These results suggest that the proposed PNC–μLED device is suitable for both display-related and VLC applications.

Background: The COVID-19 pandemic has affected all sectors of the economy and society. To understand the impact of the pandemic on firms in China and suggest responding public policies, we investigated firms in Guangdong Province (a Province with the highest Gross Domestic Product in China). Methods: The survey sample included 524 firms in 15 cities in Guangdong Province. We chose these firms from list published by the government, considering the industrial characteristics of Guangdong province and firm size. The questionnaire comprised of four categories and included 17 questions was developed based on previous studies carried out by OECD. The executives of firms were contacted by phone or WeChat, and were invited to answer self-administered questionnaires through an on-line survey platform. The data was analyzed by SPSS.Results: The following findings are worth to be noticed: (1) 48.7% of firms maintained stability, and 35.1% of the firms experienced a halt in operation or faced closure; (2) Nearly 70%-90% of the firms are or are willing to transform to online marketing, remote office work, and digital operations. (3) 46% of firms believe that there will be a certain loss this year, and 83.5% expected a decreasing trend of the city's GDP growth.Conclusions: firms in Guangdong Province have faced great challenges in the epidemic. The firms’ production and operation activities are limited, and risks are faced. It is necessary to effectively implement supporting policies to profoundly lower production costs for firms, and help firms survive the difficult period, and even gradually transit to normal business operation status.

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Background : The COVID-19 pandemic has affected all sectors of the economy and society. To understand the impact of the pandemic on firms in China and suggest the responding public policy, we investigate firms in Guangdong Province (the Province with Highest Gross Domestic Product in China).Methods : The survey sample included 524 firms in 15 cities of Guangdong Province. And we choose these firms from firm list published by government, considering the industrial characteristics of Guangdong province and firm size. The questionnaire comprised of four categories and included 17 questions was developed based on previous studies carried out by OECD. The executives of firms were contacted by phone or WeChat, and invited to answer the self-administered questionnaires through an on-line survey platform. The data was analyzed by SPSS.Results : The following findings are worth to be noticed: (1) 48.7% of firms maintained overall stability, and 35.1% of the firms experienced a halt in operation or faced closure; (2) Nearly 70%-90% of the firms are or are willing to transform to online marketing, remote office work and digital operations. (3) 46% of firms believe that there will be a certain loss this year, and 83.5% expected a decrease trend of the city's GDP growth.Conclusions : firms in Guangdong Province face great challenges in epidemic. The firms’ production and operation activities are limited, and face risks. It is necessary to effectively implement supporting policies to profoundly lower production costs for firms, and help firms survive the difficult period, even gradually transit to normal business operation status.