In this study, a monoclonal antibody with selectivity for clopidol was produced based on a new hapten. The 50% inhibition concentration (IC 50 ) for the developed ic-ELISA was 0.275 ng/mL. Cross-reactivity tests showed that the monoclonal antibody had high specificity for clopidol. Using this antibody, a lateral-flow colloidal gold immunoassay (LCGA) was created for the rapid screening of clopidol in raw chicken samples. With the naked eye, the cut-off value of the strip assay was 5 μg/kg for clopidol in chicken samples. Therefore, this LCGA provides a sensitive and rapid method for on-site detection of clopidol in chicken samples. ARTICLE HISTORY

Tert-butylhydroquinone (TBHQ) residues in foods pose a threat to human health. Therefore, it is necessary to develop a rapid method for TBHQ detection. In this study, a sensitive monoclonal antibody...

We prepared monoclonal antibodies (mAbs) against diminazene and used them in the development of a gold nanoparticle-based lateral-flow test (GNT) strip and indirect competitive enzyme-linked immunosorbent assay for the detection...