Purpose: This study examines practitioner participation over 12 years in National Dental Practice-Based Research Network (PBRN) studies and practitioner meetings, average length of participation, and association between practitioner- and practice-level characteristics and participation. Little information exists about practitioners’ long-term participation in PBRNs. Methods: The network conducted a retrospective analysis of practitioner participation in three main network activities during 2005–2017. Practitioners who completed an enrollment questionnaire, practiced in the U.S., and either attended a network meeting or received an invitation to complete a questionnaire or clinical study were included in the analysis. 3,669 practitioners met inclusion criteria. The network implemented 38 studies (28 clinical and 10 questionnaire), 23 of which (15 clinical and 8 questionnaire) met the criteria for the current analysis. Results: Overall, 86% (N=3,148) participated in at least one network activity during 2005–2017. Questionnaire studies had the highest rate with 81% (N=2,963) completing at least one, 21% (N=762) completed at least 1 clinical study and 19% (N=700) attended at least one network meeting. Among 1,578 practitioners enrolled in the first five years of the Network launch, 20% (N=320) participated in multiple network activities over 5–9 years, and 14% (N= 238) for 10–12 years. Practitioner characteristics associated with participation varied depending on the activity assessed. Conclusion: The network engaged practitioners in its research activities with relatively high participation rates over a 12-year period. Strategies employed by the network to engage practitioners may serve as a model for PBRN networks for other allied health professions.