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[a] 1Introduction Strontium Ranelate (SR)i sc hemically known as 2-(2-Carboxy-4-cyano-5-[N,N-di(carboxymethyl)amino]thiophen-3-yl) acetic acid distrontium salt [1,2].S Ri st he only anti-osteoporotic agent which both increases bone formation and reduces bone resorption, resulting in ar ebalance of bone turnover in favor of bone formation.S R is given orally in the treatment of postmenopausalo steoporosis to reduce the risk of vertebral and hip fractures [1,2].V ery few analytical techniques appeared in the literature for the determination of SR in pharmaceutical formulations.T he methods so far reported include high performance liquid chromatography HPLC with UV detection [3],s tability indicating assay studies usingR P-HPLC were also conducted [ 4,5],U Vs pectrophotometry [6,7] and capillary zone electrophoresis [8].A lso,s ome methods utilized the strontium ions for determinationo f the drug such as atomic absorption spectrophotometry AAS [9] and electrochemical detection of strontium throughi nteraction with metallothionein [10].To our knowledge,n ov oltammetric methods for the direct determination of SR have been reported. Hence, the current electroanalyticalr esearch aimed to study the DP and SW voltammetric behavior of SR in its pure and pharmaceutical dosage form. Thee lectroanalytical technique provides the advantages of simplicity,h igh sensitivity,l ow cost, relatively short analysis time and direct analysis,w ithout any derivatization, extraction or clean-up steps [11,12]. Materialsa nd ReagentsAll reagentsa nd solvents were of analytical reagent grade and used without further purification. Pure Sample and Commercial DosageF ormSR of purity 98 %: manufactured by Les laboraroires Servier Industrie -F rance.Thep harmaceutical preparation Protelos ,2 gS Rs achets:( Batch No.870544, Manuf.B yLes laboraroires Servier Industrie -F rance, Packed by Servier Egypt IndustriesL imited -6 th OctoberC ity -E gypt) wasp urchased from the local market. ReagentsBritton Robinson (BR) buffer 0.04 M, wasp repared by mixing the acid mixture containing0 .04 Mp hosphoric acid, 0.04 Ma cetic acid and 0.04 Mb oric acid [13].B uffer solutionsw ere adjusted with the appropriate amount of 0.2 Ms odium hydroxide to get the desired pH in the Abstract:T he voltammetric behavioro fS trontium Ranelate (SR) was studied using Cyclic (CV),d ifferential pulse (DPV)a nd square wave (SWV) voltammetry.C V showed twow ell-defined, irreversible,d iffusion-controlled anodicp eaks usingB ritton-Robinsonb uffer, pH 2.0 at Pencil graphite (PGE), Carbon paste (CPE) and glassy carbon (GCE)e lectrodes.T he peak currentconcentrationr elationship was rectilinearo ver the range 1.0-10.0, 1.0-11.25 and 2.5-24.0 mg/mL at PGE,C PE and GCE respectively,w ith am inimum detectability of 0.17, 0.24a nd 0.39 mg/mL for peak 1a nd 0.19,0 .27 and 0.51 mg/ mL for peak 2. Recoveries showed the high accuracy of the method; 99.8 %, 99.5 %a nd 99.7 %a tP GE, CPE and GCE respectively for peak 1a nd 100.1 %, 99.9 %a nd 99.7 %a tP GE, CPE and GCE respectively for pe...

Simple and sensitive second derivative and synchronous spectrofluorimetric methods have been developed and validated for the quantitative determination of oxytocin and ergometrine maleate in their pure and combined dosage forms. The methods are based on the derivatization reaction of oxytocin with fluorescamine reagent, which yielded a highly fluorescent compound measured at 486 nm after excitation at 390 nm. Ergometrine was directly measured in combination with oxytocin, since it exhibits native fluorescence at 421 nm after excitation at 300 nm. Quantitation of oxytocin in presence of ergometrine was also successful at 482 and 477 nm using second derivative and synchronous spectrofluorimetry, respectively. Different experimental parameters were studied and optimized. The relative fluorescence intensity versus concentration plot was rectilinear over the range of 0.04-0.75 and 5-100 ng/mL for oxytocin and ergometrine, respectively. The methods were successfully applied for the determination of both drugs in their prepared combined ampoules. The methods were validated and compared with the reference chromatographic method; they revealed good accuracy and reproducible results. The proposed methods showed high accuracy and sensitivity, with no requirement of multiple steps or previous chemical separation.Oxytocin Validation Dosage form Ergometrine maleate Synchronous spectrofluorimetry Second derivative spectrofluorimetry