ÖzBu çal›flmada 13 adet eflek sütünün kimyasal özelliklerinden kuru madde (%), ya¤ (%), pH ve titrasyon asitli¤i (˚SH) de¤erleri belirlenmifltir. Mikrobiyolojik analizlerden toplam aerobik mezofil bakteri (TAMB), laktik asit bakterileri (LAB), koliform grup bakteri, enterokok, stafilokok/ mikrokok ve Staphylococcus aureus analizleri gerçeklefltirilmifltir. Eflek sütünün protein profili Sodyum Dodesil Sulfat Poliakrilamid Jel Elektroforezi (SDS-PAGE) yöntemi ile belirlenmifltir. Eflek sütlerinde pH, titrasyon asitli¤i, kuru madde ve ya¤ de¤erleri s›ras›yla 7.16-7.62, 2.0-3.5, 8.42-9.82, 0.40-0.95 aral›klar›nda de¤iflim göstermifltir. Örneklerin toplam mezofil aerob bakteri say›s› 3.12-7.48 log kob/mL, laktobasil ve laktokok say›s› 3.35-5.58 log kob/mL, 4.3-6.93 log kob/mL, enterokok 3.55-5.01 log kob/mL, koliform grup bakteri say›s› 3.51-6.45 log kob/mL, stafilokok/mikrokok say›s› 3.53-6.31 log kob/mL olarak saptanm›flt›r. Tüm süt örneklerinde Staphylococcus aureus'a rastlanmam›flt›r. DETERMINATION OF CHEMICAL AND MICROBIOLOGICAL PROPERTIES OF DONKEY'S MILK PRODUCED IN ISPARTA AbstractIn this study, dry matter (%), fat (%), pH and titratable acidity (˚SH) values were determined from chemical properties of 13 donkey's milk. Total aerobic mesophilic bacteria (TAMB), lactic acid bacteria (LAB), coliform group bacteria, enterococci, staphylococcus/micrococcus and Staphylococcus aureus analyses were performed. Donkey's milk protein profile determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-page) method. pH, titratable acidity, dry matter and fat values ranged from 7.16 to 7.62, 2.0 to 3.25, 8.42 to 9.82, 0.40 to 0.95 respectively. The total mesophilic aerob bacteria counts of the samples were determined 3.12-7.48 log-cfu/mL, Lactobacilli and Lactococci 3.35-5.58 log-cfu/mL, 4.3-6.93 log-cfu/mL, Enterococci spp 3.55-5.01 log-cfu/mL, Coliform group bacteria counts 3.51-6.45 log-cfu/mL and Staphylococcus/micrococcus 3.53-6.31 log-cfu/mL. Staphylococcus aureus was not detected in all milk samples.