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Citrus species are among the plants that generate essential oils, which include a variety of chemical components with antibacterial properties. Citrus x aurantiifolia is one of the citrus types developed by a cross between lime (Citrus aurantifolia) and lemon (Citrus hystrix). The purpose of this study is to compare the chemical component and antibacterial activity of essential oils extracted from Citrus x aurantifolia fruit peel. Hydrodistillation was used to extract the essential oil (EO). Gas chromatography in conjunction with mass spectroscopy were used to analyze chemical composition (GC-MS). D-limonene (44.1%), Terpinen (20.9%), and camphene (14,512%) were the primary chemical components detected in the peel. The dilution method was used to determine the antibacterial activity. The antibacterial test were suppressed the growth of bacteria by this essential oil. Pseudomonas aeruginosa, Streptococcus mutans, Escherichia coli, and Methicillin Resistant Staphylococcus aureus all had MIC values of 125 mg/ml, while Staphylococcus aureus had MIC values of 62.5 mg/ml.