Diabetes mellitus (DM) is a serious health problem worldwide, World Health Organization (WHO) declared Indonesia takes the fourth place in the DM problem, to overcome this problem, a synthesis drug and medicines have been produced, but production from this medicine has side effects and require a lot of money for it, while many try to find a solution to this disease, there are plants that can help in lowering blood glucose and that is Jombang as we known as dandelion, because of this plant ability, this research is made. The purpose of this study is to obtain accurate data for lowering white rat’s blood glucose from 1 gram extract of leaf and root as determined volume is 0.4 ml, 0.6 ml, and 0.8 ml from leaf extract each contains 0.65 gram, 0.97 gram, and 1.29 gram and root extract each contains 0.77 gram, 1.15 gram, and 1.54 gram weight wet of herbs. This study uses True Experimental Design, 22 male rats are divided into five groups which each group has two positive controls, two negative controls, six for leaf extract, six for root extract, and six for combined leaf and root extracts. Treatment start from day 6 to day 15 and given two times treatment a day every 12 hour, the measurement of data was collected on day 6, 9, 12, and 15. The data obtained is analyzed with logistic regression, ANOVA multivariate followed by Duncan test. The result showed that the volume of group leaf extract treatment 0.4 ml (0.65 gram), 0.6 ml (0.97 gram), and 0.8 ml (1.29 ml) has a value α = 0.007, α = 0.016, and α = 0.009, root extract 0.4 ml (0.77 gram), 0,6 ml (1.15 gram) has a value α = 0.001 and α = 0.008 , and mixture of leaf and root extracts of 0.4 ml (1.42 gram) has a value of α = 0.005 at 95% trusted stage, at the end of the study, the best volume of extract in lowering blood glucose is 0.4 ml (1.42 gram) mixture of leaf and root extracts of dandelion.
