An epithelial cell adhesion molecule (EpCAM) was selectively expressed in human colorectal carcinoma. Treatment with plant-derived anti-EpCAM mAb (mAbP CO17-1A) and RAW264.7 cells inhibited cell growth in the human colorectal cancer cell line SW620. In SW620 treated with mAbP CO17-1A and RAW264.7 cells, expression of p53 and p21 increased, whereas the expression of G1 phase-related proteins, cyclin D1, CDK4, cyclin E, and CDK2, decreased, similar to mammalian-derived mAb (mAbM) CO17-1A. Similar to mAbM CO17-1A, treatment with mAbP CO17-1A and RAW264.7 cell decreased the expression of anti-apoptotic protein, Bcl-2, but the expression of pro-apoptotic proteins Bax, TNF-α, caspase-3, caspase-6, caspase-8 and caspase-9, increased. Cells treated with mAbP CO17-1A and RAW264.7 cells expressed metastasis-related gangliosides, GM1 and GD1a, similar to mAbM CO17-1A. These results suggest that mAbP CO17-1A is as effective on anti-cancer activity as mAbM CO17-1A.

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<p>Currently vehicle traffic regulations regarding on axle and total weights in Korea are simple, outdated and different from the regulations in other countries, such as US or European countries. In this study, load effect of over-loaded and special permit vehicle traffic on bridges and pavements are analyzed. Types of typical bridges include concrete and steel girder bridges. Types of pavements include asphalt and cement concrete pavements. Various weigh-in-motion (WIM) truck data are collected and used for comparing overweight ratios. Based on WIM truck data, load effects of various oversize vehicles mixed with common traffic are analyzed for various bridge types. Oversize vehicles include vehicles used in construction field, crane vehicles and transport vehicles for the military purpose. The effect of axle types on pavement design is also analyzed. The results of this study will be the basis of new provisions and regulations regarding on axle and total weights limitations as well as special permit vehicle.</p>

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