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Journal of Digital Convergence 501 E f f e c t so fL a u g h t e rT h e r a p yo nDe p r e s s i o ni ne l d e r l yMiYo u n gCh a n g Gwa n g j uUn i v e r s i t y 요 약 본 연구는 웃음요법이 노인의 우울에 미치는 효과를 규명하고자 시도하였다.본 연구는 유사 실험 설계로 비동등성 대조군 전후 실험 설계이며,경로당을 이용하는 6 5 세 이상 노인 3 8 명을 대상으로 하였다.자료수집 기간은 2 0 1 3 년 1 0 월 1 일부터 1 0 월 2 2 일까지이며 실험 군( n = 1 9 명) 은 4 주간 매주 1 회씩 5 0 분간 웃음치료 프로그램에 참여하 였고 프로그램 전 후 GDS S F -K를 이용하여 우울을 측정하였다.자료 분석은 χ 2 -t e s t ,t -t e s t ,Ma n n -Wh i t n e y t e s t , Wi l c o x o ns i g n e dr a n kt e s t 를 이용하여 분석하였다.본 연구결과 웃음치료 프로그램은 노인의 우울을 유의하게 감소시 키는 효과가 있었다( z = -2 . 5 9 ,p = . 0 1 7 ) .따라서 본 연구의 웃음치료프로그램은 지역사회 노인 간호 실무에서 활용 가 능하며 노인의 우울관련 간호중재 전략 수립에 유용하게 활용될 수 있을 것으로 기대된다. 주제어 : 노인,우울,웃음치료,간호중재,지역사회Abstract Purpose: The purpose of this study was to examine the effect of a laughter therapy on depression, of elderly. Methods: A quasi-experimental, nonequivalent control group pretest posttest design was used. Participants (n=38) included elders. Data were collected from October 1 to 22, 2013. Experimental group (n=19) participated in laughter therapy four times, once a week for 50 min per session. Questionnaires were used to measure pretest and posttest levels of depression. Results: At the end of four-week intervention, depression score (z=-2.59, p= .017) was significantly decreased in the experimental group compared to control group. Conclusion: The study results suggest that the laughter therapy is an effective intervention to reduce the depression. It seems that this program can widely be utilized as one of nursing intervention programs for the elderly.Key Words : aged people, depression, laughter therapy, nursing practice.Re c e i v e d1 9Ap r i l2 0 1 4 ,Re v i s e d2 1Ma y2 0 1 4 Ac c e p t e d2 0J u n e2 0 1 4 C o r r e s p o n d i n gAu t h o r :MiYo u n gC h a n g ( Gwa n g j uUn i v e r s i t y ) E ma i l :my c h a n g @g wa n g j u . a c . k r Ⓒ Th e S o c i e t y o fDi g i t a lP o l i c y & Ma n a g e me n t .Al lr i g h t s r e s e r v e d .Th i si sa n o p e n -a c c e s sa r t i c l ed i s t r i b u t e du n d e rt h e t e r mso ft h eC r e a t i v eC o mmo n sAt t r i b u t i o n No n -C o mme r c i a l L i c e n s e( h t t p : / / c r e a t i v e c o mmo n s . o r g / l i c e n s e s / b y -n c / 3 . 0 ) ,wh i c h p e r mi t s u n r e s t r i c t

To maintain embryogenic cell lines of Pimpinella brachycarpa, we suspension-cultured friable and rapidly growing yellowish calli in an MS liquid medium containing 0.2 ~ 2,4-D and 0.5 pM BAP. Efficient somatic embryogenesis was achieved when selected cells were then transferred to an MS medium (0.2% gelrite) that contained 0.2 gM 2,4-D, 0.5 ~4 BAP, and 10.0 laM TDZ (thidiazuron). These cells were cultured at 27~ under continuous illumination (21.5 I~E m -2 s-l). Embryogenic calli expanded about four-fold, and developed into pale yellow calli. Somatic embryogenesis was initiated only from glossy and nodular-type calli. After two more weeks of culture, globular embryos appeared on the surface of calli grown in the MS medium that contained 10.0/aM TDZ only, or in combination with 0.5 gM NAA. Experimenting with 2,4-D, an auxin, to promote embryogenic calli resulted in excessive browning and death. We overcame this problem by growing glossy embryogenic and nodular calli on media that contained 10.0 gM TDZ. Calli that were not treated with TDZ turned dark brown and were not viable. Up to 74% of the calli showed somatic embryos when the medium was supplemented with 10.0 gM TDZ and 0.5 I~1 NAA. Embryos from these TDZinduced, somatic embryogenic calli grew efficiently, forming multiple shoots and developing into normal plants. Therefore, efficient differentiation of suspension-cultured cell clusters into embryogenic calli, along with treatment of subsequent somatic embryos by TDZ, suggests that TDZ probably helps in establishing the optimum cytokinin-auxin ratio required for induction and expression of somatic embryogenesis.

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